ENPP3 / CD203c Recombinant Protein
| Code | Size | Price |
|---|
| PSI-11-147-0.1mg | 0.1mg | £729.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Shipping:
blue ice or RT
Storage:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
ENPP3,CD203c,NPP3,E-NPP3,PD-Ibeta,NPPase,PDNP3
Application Note:
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 96.9 kDa. The protein migrates as 115-120 kDa and 121-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Background:
The human NPP family contains seven members which can be classified into two groups according to their substrate preferences. The first class comprises the nucleotide-degrading proteins NPP1, 3 and 4. NPP3 (CD203c, ENPP3) is expressed in multiple organs, including on epithelial and mucosal surfaces, and notably on basophils and mast cells. Activation of basophils by antigen-bound IgE leads to release of inflammatory mediators and rapid upregulation of NPP3 to the cell surface. This protein is in fact a common marker for diagnosing allergen sensitivity with patient basophils by flow cytometry. Basophils and mast cells mediate the response to certain pathogens, as well as acute and chronic allergic reactions. Following activation, these cells release ATP, which further stimulates them in an autocrine manner. NPP3 upregulation serves to degrade ATP and suppress chronic allergic inflammation (but not the acute response).
Buffer:
50 mM Tris, 150 mM NaCl, pH8.0
Fusion Tag:
His Tag
NCBI Gene ID #:
5169
NCBI Official Name:
ectonucleotidepyrophosphatase/phosphodiesterase3
NCBI Official Symbol:
ENPP3
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
96.9 kDa
Protein Accession #:
NP_005012.2
Purity:
>90% as determined by SDS-PAGE.
Recombinant Protein Sequence:
#N/A
Source:
HEK293 cells
Swissprot #:
O14638