PSGL-1 / CD162 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-11-244-0.1mg | 0.1mg | £729.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Shipping:
blue ice or RT
Storage:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
PSGL1,CD162,SELPLG,Selectin P ligand
Application Note:
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 30.7 kDa. The protein migrates as 55-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Background:
P-selectin glycoprotein ligand 1 (PSGL-1) is also known as Selectin P ligand (SELPLG), CD antigen CD162. PSGL-1 is disulfide-linked homodimer which is the high affinitycounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 / SELPLG interacts with P-, E- and L-selectins, through their lectin/EGF domains, is required for promoting recruitment and rolling of leukocytes. These interactions require sialyl Lewis X glycan modification but there is a differing dependence for tyrosine sulfations. Dimerization appears not to be required for P-selectin/SELP binding. PSGL-1 can interact with SNX20, MSN and SYK. PSGL-1 / SELPLG mediate the activation of SYK by SELPLG.
Background References:
- Rodgers S.D., et al., 2001, Biophys. J. 81:2001-2009.
- Bernimoulin M.P., et al., 2003, J. Biol. Chem. 278:37-47.
- Somers W.S., et al., 2000, Cell 103:467-479.
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
6404
NCBI Official Name:
selectin P ligand
NCBI Official Symbol:
SELPLG
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
30.7 kDa
Protein Accession #:
NP_002997.2
Purity:
>90% as determined by SDS-PAGE.
Recombinant Protein Sequence:
#N/A
Source:
HEK293 cells
Swissprot #:
Q14242