Siglec-8 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-11-319-0.1mg | 0.1mg | £729.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Shipping:
blue ice or RT
Storage:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
Siglec-8,SAF-2,SIGLEC8,SAF2
Application Note:
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 39.7 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Background:
Siglec-8 is also known as SIGLEC8, SAF2, SIGLEC-8, SIGLEC8L and sialic acid binding Ig like lectin 8. Siglec-8 was first identified by CD33 homology screening of ESTs from a cDNA library generated from a patient diagnosed with idiopathic hypereosinophilic syndrome and was originally termed SAF-2 (sialoadhesin family 2). At the tissue level, Siglec-8 mRNA was found to be most highly expressed in lung, PBMCs, spleen, and kidney. Two splice variants of Siglec-8 exist. The initially characterized form contains 431 amino acid residues in total, subsequently, a longer form of Siglec-8, initially termed Siglec-8L. Both forms of Siglec-8 are found in eosinophils and contain a V-set domain with lectin activity and two C2-type Ig repeat domains in the extracellular region.
Background References:
- Floyd H, et al. 2000. J Biol Chem. 275(2):861-6.
- Foussias G, et al. 2000. Biochem Biophys Res Commun. 278(3):775-81.
- Kikly KK, et al. 2000. J Allergy Clin Immunol. 105(6 Pt 1):1093-100.
Buffer:
PBS?with?Arginine,?pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
2817
NCBI Official Name:
glypican1
NCBI Official Symbol:
GPC1
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
39.7 kDa
Protein Accession #:
NP_002072.2
Purity:
>95% as determined by SDS-PAGE.
Recombinant Protein Sequence:
#N/A
Source:
HEK293 cells
Swissprot #:
P35052