PSMA / FOLH1 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-11-228-0.1mg | 0.1mg | £838.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Shipping:
blue ice or RT
Storage:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
FOLH1,PSMA,GIG27,FOLH,NAALAD1,PSM,NAALADase I,GCPII,FGCP
Application Note:
This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 106.0 kDa. The protein migrates as 110-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Background:
Prostate-specific membrane antigen (PSMA) is also known as Folate hydrolase 1 (FOLH1), Glutamate carboxypeptidase 2 (GCP2), N-acetylated-alpha-linked acidic dipeptidase I (NAALAD1), which belongs to the peptidase M28 family and M28B subfamily. FOLH1 / PSMA is stable at pH greater than 6.5. FOLH1 / PSMA is a type II transmembrane zinc metallopeptidase that is most highly expressed in the nervous system, prostate, kidney, and small intestine. FOLH1 / GCP-2 is homodimer and binds 2 zinc ions per subunit, and required for NAALADase activity. The catalytic activity of PSMA involved in releasing of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly ? gamma - glutamates. FOLH1 / GCP-2 / PSMA has both folate hydrolase and N ? acetylated ? alpha ? linked - acidic dipeptidase (NAALADase) activity and has a preference for tri-alpha-glutamate peptides. GCP-2 / PSMA involved in prostate tumor progression and also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
Background References:
- Grauer L.S., et al., 1998, Cancer Res. 58:4787-4789.
- Mesters J.R., et al., 2006, EMBO J. 25:1375-1384.
- Barinka C., et al., 2009, J. Med. Chem. 50:3267-3273.
Buffer:
50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5
Fusion Tag:
Fc Tag
NCBI Gene ID #:
53320
NCBI Official Name:
folatehydrolase1
NCBI Official Symbol:
Folh1
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Predicted Molecular Weight:
106.0 kDa
Protein Accession #:
NP_058050.3
Purity:
>95% as determined by SDS-PAGE.
Recombinant Protein Sequence:
#N/A
Source:
HEK293 cells
Swissprot #:
O35409