Insulin R / CD220 (28-944) Recombinant Protein
| Code | Size | Price |
|---|
| PSI-11-371-0.1mg | 0.1mg | £657.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Shipping:
blue ice or RT
Storage:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
INSR,Insulin receptor,IR,CD220
Application Note:
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 106.5 kDa. The protein migrates as 115-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Background:
Insulin receptor (INSR) is also known as CD antigen CD220, which can be cleaved into the following 2 chains: Insulin receptor subunit alpha and Insulin receptor subunit beta. INSR is a tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains carry the insulin-binding regions, while the beta chains carry the kinase domain. Forms a hybrid receptor with IGF1R, the hybrid is a tetramer consisting of 1 alpha chain and 1 beta chain of INSR and 1 alpha chain and 1 beta chain of IGF1R. In addition to binding insulin, the insulin receptor can bind insulin-like growth factors (IGFI and IGFII). Isoform Short of INSR has a higher affinity for IGFII binding. When present in a hybrid receptor with IGF1R, INSR binds IGF1.
Background References:
- Pandini G., et al., 2002, J. Biol. Chem. 277:39684-39695.
- Slaaby R., et al., 2006, J. Biol. Chem. 281:25869-25874.
- Menting J.G., et al., 2013, Nature 493:241-245.
- Kadowaki T., et al., 1988, Science 240:787-790.
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
3643
NCBI Official Name:
insulin receptor
NCBI Official Symbol:
INSR
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
106.5 kDa
Protein Accession #:
NP_000199.2
Purity:
>90% as determined by SDS-PAGE.
Recombinant Protein Sequence:
#N/A
Source:
HEK293 cells
Swissprot #:
P06213