LGR5?/?GPR49 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-11-137-0.1mg | 0.1mg | £729.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Shipping:
blue ice or RT
Storage:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
LGR5, GPR49, GPR67, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, Leucine-rich repeat-containing G-protein coupled receptor 5
Application Note:
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 62.0 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Background:
LGR5 (also known as GPR49) is a seven-transmembrane protein of the class A Rhodopsin-like family of GPCRs. LGR5 and LGR4 bind the R-spondins with high affinity and mediate the potentiation of Wnt/β-catenin signaling by enhancing Wnt-induced LRP6 phosphorylation. The LGR5/RSPO complex could promote Wnt signalling via the neutralization of two transmembrane E3 ligases, RNF43 and ZNRF3.
Background References:
- Morgan RG. et al. Br J Cancer. 2018 May 29; 118(11): 1410?1418.
- Yue Z. et al. FASEB J. 2018 May;32(5):2422-2437.
- de Lau W. et al. Genes & development. 2014;28:305?16.
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
8549
NCBI Official Name:
leucinerichrepeatcontainingGprotein-coupledreceptor5
NCBI Official Symbol:
LGR5
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
62.0 kDa
Protein Accession #:
NP_003658.1
Purity:
>90% as determined by SDS-PAGE.
Recombinant Protein Sequence:
#N/A
Source:
HEK293 cells
Swissprot #:
O75473