TREM2 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-11-171-0.1mg | 0.1mg | £729.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Shipping:
blue ice or RT
Storage:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
Triggering receptor expressed on myeloid cells 2/TREM2/TREM-2
Application Note:
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 19.3 kDa. The protein migrates as 26-40 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Background:
Triggering receptor expressed on myeloid cells 2 (TREM2) is a cell surface receptor of the immunoglobulin superfamily. The TREM2 is found in various tissue macrophages, such as CNS microglia, bone osteoclasts, alveolar, peritoneal and intestinal macrophages. TREM2 is also present on cultured bone marrow-derived macrophages and monocyte-derived dendritic cells. Some research have identified a rare variant of TREM2 that is a risk factor for Alzheimer disease (AD), which is the most common form of late-onset dementia.The extracellular region of TREM2 contains a single immunoglobulin superfamily domain and binds polyanionic ligands, such as bacterial lipopolysaccharide (LPS) and phospholipids8. Upon ligand binding, TREM2 transmits intracellular signals through an adaptor, DAP12 (also known as TYRO protein tyrosine kinase-binding protein (TYROBP)), which is associated with the transmembrane region of TREM2 and which recruits the protein tyrosine kinase SYK through its cytosolic immunoreceptor tyrosine-based activation motifs (ITAMs). TREM2 is a pro-tumorigenic marker of tumor-infiltrating macrophages in mouse models and human tumors that can be targeted to curb tumor growth and improve the efficacy of checkpoint blockade therapy while remodeling the landscape of tumor-infiltrating macrophages.
Background References:
- Martina Molgora, Ekaterina Esaulova et al. TREM2 Modulation Remodels the Tumor Myeloid Landscape Enhancing Anti-PD-1 Immunotherapy.[J]. CELL, 2020.
- Colonna M , Wang Y . TREM2 variants: new keys to decipher Alzheimer disease pathogenesis.[J]. Nature reviews Neuroence, 2016.
- Karanfilian L , Tosto M G , Malki K . The Role of TREM2 in Alzheimer's Disease; Evidence from Transgenic Mouse Models[J]. Neurobiology of Aging, 2019.
- Guerreiro R , Wojtas A , Bras J , et al. TREM2 variants in Alzheimer's disease.[J]. New England Journal of Medicine, 2013, 368(2).
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
54209
NCBI Official Name:
triggering receptor expressed on myeloid cells 2
NCBI Official Symbol:
TREM2
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
19.3 kDa
Protein Accession #:
NP_061838.1
Purity:
>90% as determined by SDS-PAGE.
Recombinant Protein Sequence:
#N/A
Source:
HEK293 cells
Swissprot #:
Q9NZC2