IGF-I R / CD221 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-11-445-0.1mg | 0.1mg | £729.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Shipping:
blue ice or RT
Storage:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
IGF1R,IGFR,JTK13,CD221,MGC142170,MGC142172,MGC18216
Application Note:
This protein carries a polyhistidine tag at the C-terminus.
This protein contains a furin convertase cleavage site, 738-RRRR-741, and will be partially processed into N (α chain) and C-terminal fragment (partial β chain) with calculated MW of 81.3 kDa and
Background:
The Insulin-like Growth Factor 1 Receptor (IGF1) is also known as CD221, JTK13. and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF1R is make up of two alpha subunits and two beta subunits ,the Both the α and β subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane αβ chain.The α chains are located extracellularly while the β subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF1R have a binding site for ATP, which is used to provide the phosphates for autophosphorylation. There is a 60% homology between IGF1R and the insulin receptor. In response to ligand binding, the α chains induce the tyrosine autophosphorylation of the β chains. This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation.
Background References:
- Jones JI, Clemmons DR., 1995, Endocr. Rev. 16 : 3?34.
- LeRoith D, et al., 1995, Endocr. Rev. 16 (2): 143?63.
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
16001
NCBI Official Name:
insulin-like growth factor I receptor
NCBI Official Symbol:
Igf1r
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Predicted Molecular Weight:
81.3 kDa, 24.3 kDa
Protein Accession #:
NP_034643.2
Purity:
>90% as determined by SDS-PAGE.
Recombinant Protein Sequence:
#N/A
Source:
HEK293 cells
Swissprot #:
Q60751