SIRP alpha / CD172a Recombinant Protein
| Code | Size | Price |
|---|
| PSI-11-506-0.1mg | 0.1mg | £657.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Shipping:
blue ice or RT
Storage:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
SHPS1,SIRPA,CD172A,BIT,MFR,MYD1,P84,PTPNS1
Application Note:
This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 65.0 kDa. The protein migrates as 90-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Background:
Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) is also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. SIRP alpha is Ubiquitous and highly expressed in brain. SIRPA / CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA / SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. SIRPA / MyD1 involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin and mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
Background References:
- Timms J.F., et al., 1999, Curr. Biol. 9:927-930.
- Stofega M.R., et al., 2000, J. Biol. Chem. 275:28222-28229.
- Latour S., et al., 2001, J. Immunol. 167:2547-2554.
Buffer:
50 mM Tris, 100 mM Glycine, pH7.5
Fusion Tag:
Mouse IgG Fc Tag
NCBI Gene ID #:
19261
NCBI Official Name:
signal-regulatory protein alpha
NCBI Official Symbol:
Sirpa
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Predicted Molecular Weight:
65.0 kDa
Protein Accession #:
BAA13520.1 (G365D)
Purity:
>95% as determined by SDS-PAGE.
Recombinant Protein Sequence:
#N/A
Source:
HEK293 cells
Swissprot #:
P97797