Complement C5 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-11-259-0.1mg | 0.1mg | £729.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Target Species: Rabbit
Application: Western Blot (WB)
Shipping:
blue ice or RT
Storage:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
Complement?C5,C5,CPAMD4
Application Note:
This protein carries a polyhistidine tag at the C-terminus.
The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. During cultivation, Complement C5 will be partially processed into α and β c
Background:
Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain). Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
Background References:
- DeMartino JA, et al. 1994. J Biol Chem. 269(20):14446-50.
- Fredslund F, et al. 2008. Nat Immunol. 9(7):753-60.
- Chen Z, et al. 1998. J Biol Chem. 273(17):10411-9.
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
103352022
NCBI Official Name:
complementC5
NCBI Official Symbol:
C5
NCBI Organism:
Oryctolagus cuniculus
Physical State:
Lyophilized
Predicted Molecular Weight:
73.8 kDa (β chain) & 111.9 kDa (α chain)
Protein Accession #:
XP_008271599.1
Purity:
>90% as determined by SDS-PAGE.
Recombinant Protein Sequence:
#N/A
Source:
HEK293 cells