CBLB / RNF56 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-11-318-0.1mg | 0.1mg | £729.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: E.coli
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Shipping:
blue ice or RT
Storage:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
CBL-B,CBLB,RNF56
Application Note:
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 47.0 kDa. The protein migrates as 46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Background:
Casitas B-lineage lymphoma proto-oncogene b (CBLB) is also known as RNF56. E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. Slightly promotes SRC ubiquitination. May be involved in EGFR ubiquitination and internalization. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. Expressed in placenta, heart, lung, kidney, spleen, ovary and testis, as well as fetal brain and liver and hematopoietic cell lines, but not in adult brain, liver, pancreas, salivary gland, or skeletal muscle.
Background References:
- Xiao Y., et al., 2015, J Immunol., 195 (9): 4218-4227.
- Desai SS., et al., 2015, J Biol Chem., 290 (42): 25595-25608.
- Van de Veerdonk FL., et al., 2016, Nat Med., 22 (8): 834-835.
Buffer:
40 mM HEPES, 120 mM NaCl, pH7.5
Fusion Tag:
His Tag
NCBI Gene ID #:
868
NCBI Official Name:
Cblproto-oncogeneB
NCBI Official Symbol:
CBLB
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
47.0 kDa
Protein Accession #:
NP_733762.2
Purity:
>95% as determined by SDS-PAGE.
Recombinant Protein Sequence:
#N/A
Source:
E. coli
Swissprot #:
Q13191