Alpha-Synuclein Recombinant Protein
| Code | Size | Price |
|---|
| PSI-11-240-0.2mg | 0.2mg | £469.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: E.coli
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Shipping:
blue ice or RT
Storage:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
SNCA,NACP,PARK1,alpha-Synuclein
Application Note:
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.3 kDa. The protein migrates as 19 kDa under reducing (R) condition (SDS-PAGE).
Background:
Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca2+ release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity.
Background References:
- Butler B, et al. 2015. J Biol Chem. 290(49):29542-54.
- Logan T, 2017. Nat Neurosci. 20(5):681-689.
- Huang CC, et al. 2018. J Cell Sci. 131(23).
- Burr? J, et al. 2010. Science. 329(5999):1663-7.
Buffer:
100 mM NaAC, pH7.0
Fusion Tag:
His Tag
NCBI Gene ID #:
6622
NCBI Official Name:
synucleinalpha
NCBI Official Symbol:
SNCA
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
16.3 kDa
Protein Accession #:
NP_000336.1
Purity:
>90% as determined by SDS-PAGE.
Recombinant Protein Sequence:
#N/A
Source:
E. coli
Swissprot #:
P37840