HCoV-229E Nucleocapsid Recombinant Protein
| Code | Size | Price |
|---|
| PSI-11-163-0.1mg | 0.1mg | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: E.coli
Regulatory Status: RUO
Target Species: Coronavirus
Application: Western Blot (WB)
Shipping:
blue ice or RT
Storage:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
Nucleocapsid protein,NP,Protein N,COVID-19
Application Note:
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 44.4 kDa. The protein migrates as 55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Background:
Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
Background References:
- Reuter M, et al. Virus Genes. 2018. 54:5-16.
- Grunewald ME, et al. Virology. 2018. 517:62-68.
- Jeeva S, et al. PLoS One. 2017. 12(9):e0184935.
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
Physical State:
Lyophilized
Predicted Molecular Weight:
44.4 kDa
Purity:
>90% as determined by SDS-PAGE.
Recombinant Protein Sequence:
#N/A
Source:
E. coli