Fluorescein dioctanoate
| Code | Size | Price |
|---|
| CDX-F0087-M100 | 100 mg | £157.00 |
Quantity:
| CDX-F0087-M250 | 250 mg | £304.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
AMBIENT
Storage:
+4°C
Documents
Further Information
Alternate Names/Synonyms:
FDO; Fluorescein dicaprylate; 3-Oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3',6'-diyl ester octanoic acid
Appearance:
Off-white low melting solid.
CAS:
19722-86-2
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Protect from light and moisture.
InChi:
InChI=1S/C36H40O7/c1-3-5-7-9-11-17-33(37)40-25-19-21-29-31(23-25)42-32-24-26(41-34(38)18-12-10-8-6-4-2)20-22-30(32)36(29)28-16-14-13-15-27(28)35(39)43-36/h13-16,19-24H,3-12,17-18H2,1-2H3
InChiKey:
UWZNXQHGTKMTSX-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 19722-86-2. Formula: C36H40O7. MW: 584.72. Fluorescein dioctanoate is a non-fluorescent hydrophobic fluorescein derivative that can pass through the cell membrane whereupon intracellular esterases hydrolyze the dioctanoate group producing the highly fluorescent product fluorescein. The fluorescein molecules accumulate in cells that possess intact membranes so the green fluorescence can be used as a marker of cell viability. Cells that do not possess an intact cell membrane or an active metabolism may not accumulate the fluorescent product and therefore do not exhibit green fluorescence. Fluorescein dioctanoate may be used in combination with PI staining as the non-viable cells take up the PI and stain dead cells red whereas viable cells do not take up the PI and should only stain green. Fluorescein dioctanoate is a fluorogenic substrate for C8 esterase that generates green fluorescence upon esterase cleavage. Spectral Data: lambdaEx 497nM, lambdaEm 516nm. Extinction coefficient: 80000 cm-1 M-1.
Molecular Formula:
C36H40O7
Molecular Weight:
584.72
Package Type:
Vial
Product Description:
Fluorescein dioctanoate is a non-fluorescent hydrophobic fluorescein derivative that can pass through the cell membrane whereupon intracellular esterases hydrolyze the dioctanoate group producing the highly fluorescent product fluorescein. The fluorescein molecules accumulate in cells that possess intact membranes so the green fluorescence can be used as a marker of cell viability. Cells that do not possess an intact cell membrane or an active metabolism may not accumulate the fluorescent product and therefore do not exhibit green fluorescence. Fluorescein dioctanoate may be used in combination with PI staining as the non-viable cells take up the PI and stain dead cells red whereas viable cells do not take up the PI and should only stain green. Fluorescein dioctanoate is a fluorogenic substrate for C8 esterase that generates green fluorescence upon esterase cleavage. Spectral Data: lambdaEx 497nM, lambdaEm 516nm. Extinction coefficient: 80000 cm-1 M-1.
Purity:
>98%
SMILES:
O=C(O1)C2=C(C=CC=C2)C31C4=CC=C(OC(CCCCCCC)=O)C=C4OC5=C3C=CC(OC(CCCCCCC)=O)=C5
Solubility Chemicals:
Soluble in DMSO (5mg/ml).
Source / Host:
Synthetic
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at +4°C.
References
(1) J.J. Thomas, et al.; Exp. Biol. Med. 140, 179 (1972) | (2) F.-Y. Ge, et al.; Dyes Pigm. 72, 322 (2007) | (3) M. Poznika & B. Koeniga; Org. Biomol. Chem. 12, 3175 (2014)