Calcein-AM Solution
| Code | Size | Price |
|---|
| CDX-C0658-M001 | 1 mg | £450.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
BLUE ICE
Storage:
-20°C
Documents
Further Information
Alternate Names/Synonyms:
CAL-AM; Calcein Acetoxymethyl ester; NSC 689290
Appearance:
Liquid.
CAS:
148504-34-1
Concentration:
1mM in DMSO (1 mg in 1 ml DMSO)
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Protect from light and moisture.
InChi:
InChI=1S/C46H46N2O23/c1-25(49)60-21-64-41(55)17-47(18-42(56)65-22-61-26(2)50)15-31-11-35-39(13-37(31)68-29(5)53)70-40-14-38(69-30(6)54)32(12-36(40)46(35)34-10-8-7-9-33(34)45(59)71-46)16-48(19-43(57)66-23-62-27(3)51)20-44(58)67-24-63-28(4)52/h7-14H,15-24H2,1-6H3
InChiKey:
BQRGNLJZBFXNCZ-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 148504-34-1. Formula: C46H46N2O23. MW: 994.86. Calcein AM is a non-fluorescent cell permeable and hydrophobic probe that, upon entering live cells, is cleaved by intracellular esterases, releasing the membrane-impermeable, hydrophilic, and intensely bright green fluorescent calcein. Better retained by viable cells than fluorescein, carboxyfluorescein, or BCECF, due to its low cytotoxicity. It can be used as an indicator of cell viability (dead cells lack cytoplasmic esterases), cell-cell communication, cytotoxicity or changes in intracellular calcium, fluoride, iron or mercury. Calcein-AM is a neutral substrate for multidrug resistance-associated protein 1 (MRP1) and multidrug resistance protein 3 (MDR3, P-glycoprotein 3) and has been used in flow cytometry studies to analyze the function of P-gp and MRP. Used as a cytosolic fluorophore in mitochondrial permeability studies to image pore transition. Viability assays using calcein are reliable and correlate well with the standard 51Cr-release assay. Spectral Data: lambdaEx 496nm, lambdaEm 516nm in 0.1M Tris pH 8.0.
MDL:
MFCD05861516
Molecular Formula:
C46H46N2O23
Molecular Weight:
994.86
Package Type:
Vial
Product Description:
Calcein AM is a non-fluorescent cell permeable and hydrophobic probe that, upon entering live cells, is cleaved by intracellular esterases, releasing the membrane-impermeable, hydrophilic, and intensely bright green fluorescent calcein. Better retained by viable cells than fluorescein, carboxyfluorescein, or BCECF, due to its low cytotoxicity. It can be used as an indicator of cell viability (dead cells lack cytoplasmic esterases), cell-cell communication, cytotoxicity or changes in intracellular calcium, fluoride, iron or mercury. Calcein-AM is a neutral substrate for multidrug resistance-associated protein 1 (MRP1) and multidrug resistance protein 3 (MDR3, P-glycoprotein 3) and has been used in flow cytometry studies to analyze the function of P-gp and MRP. Used as a cytosolic fluorophore in mitochondrial permeability studies to image pore transition. Viability assays using calcein are reliable and correlate well with the standard 51Cr-release assay. Spectral Data: lambdaEx 496nm, lambdaEm 516nm in 0.1M Tris pH 8.0.
Purity:
>95% (HPLC)
SMILES:
O=C(O1)C2=C(C=CC=C2)C31C4=CC(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(OC(C)=O)C=C4OC5=C3C=C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OC(C)=O)=C5
Solubility Chemicals:
Soluble in DMSO.
Source / Host:
Synthetic
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at -20°C.
References
(1) N. Feller, et al. Br. J. Cancer. 72, 543 (1995) | (2) N. Feller, et al. FEBS Lett. 368 385 (1995) | (3) F. Tiberghien & F. Loor, Anti-Cancer Drugs 7, 568 (1996) | (4) P. Decherchi, et al. J. Neurosci. Methods 71, 205 (1997) | (5) M. Essodaigui, et al. Biochemistry 37, 2243 (1998) | (6) J.J. Lemasters, et al. Biochim. Biophys. Acta. 1366, 177 (1998) | (7) J. Hynes, et al. J. Biomol. Screen. 8, 264 (2003) | (8) D. Bratosin, et al. Cytometry A 66, 78 (2005) | (9) R.W. Sabnis, Handbook of biological dyes and stains (2010) | (10) G. Szakacs, et al. Pathol. Oncol. Res. 4, 251 (2016)