Dibenzylfluorescein
| Code | Size | Price |
|---|
| CDX-D0282-M100 | 100 mg | £194.00 |
Quantity:
| CDX-D0282-M500 | 500 mg | £499.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
AMBIENT
Storage:
-20°C
Documents
Further Information
Alternate Names/Synonyms:
DBF; NSC 645658
Appearance:
Faint to dark orange powder.
CAS:
97744-44-0
EClass:
32160000
Form (Short):
liquid
GHS Symbol:
GHS07
Handling Advice:
Protect from light and moisture.
Hazards:
H302-H312-H332
InChi:
InChI=1S/C34H24O5/c35-25-15-17-29-31(19-25)39-32-20-26(37-21-23-9-3-1-4-10-23)16-18-30(32)33(29)27-13-7-8-14-28(27)34(36)38-22-24-11-5-2-6-12-24/h1-20H,21-22H2
InChiKey:
YZJGKSLPSGPFEV-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 97744-44-0. Formula: C34H24O5. MW: 512.55. Dibenzylfluorescein is a fluorogenic probe that acts as a substrate for specific cytochrome P450 (CYP) isoforms, including CYP3A4, CYP2C8, CYP2C9, CYP2C19 and aromatase (CYP19). It is dealkylated by these CYP isoforms to produce fluorescein benzyl ether, which is further hydrolyzed to fluorescein by the addition of base (typically 2 M NaOH). Dibenzylfluorescein is typically used near its apparent Km value of 0.87-1.9 µM. The fluorescence of fluorescein is evaluated using excitation/emission wavelengths of 485/538nm. Dibenzylfluorescein is used to detect changes in CYP catalytic activity caused by drugs or disease.
MDL:
MFCD13184323
Molecular Formula:
C34H24O5
Molecular Weight:
512.55
Package Type:
Vial
Precautions:
P280
Product Description:
Dibenzylfluorescein is a fluorogenic probe that acts as a substrate for specific cytochrome P450 (CYP) isoforms, including CYP3A4, CYP2C8, CYP2C9, CYP2C19 and aromatase (CYP19). It is dealkylated by these CYP isoforms to produce fluorescein benzyl ether, which is further hydrolyzed to fluorescein by the addition of base (typically 2 M NaOH). Dibenzylfluorescein is typically used near its apparent Km value of 0.87-1.9 µM. The fluorescence of fluorescein is evaluated using excitation/emission wavelengths of 485/538nm. Dibenzylfluorescein is used to detect changes in CYP catalytic activity caused by drugs or disease.
Purity:
>98% (NMR)
Signal word:
Warning
SMILES:
O=C(OCC1=CC=CC=C1)C2=CC=CC=C2C(C3=CC=C(OCC4=CC=CC=C4)C=C3O5)=C6C5=CC(C=C6)=O
Solubility Chemicals:
Soluble in DMSO (10mg/ml) or DMF (20mg/ml).
Source / Host:
Synthetic
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at -20°C.
References
(1) D.M. Stresser, et al.; Drug Metab. Dispos. 28, 1440 (2000) | (2) M.T. Donato, et al.; Drug Metab. Dispos. 32, 699 (2004) | (3) E.R. Troesken, et al.; Toxicol. 219, 33 (2006) | (4) K.A. Salminen, et al.; Drug Metab. Dispos. 39, 412 (2011) | (5) D. Moutinho, et al.; Drug Metab. Dispos. 40, 754 (2012)