Fura-2 pentapotassium salt

Fura-2 . 5K

Off-white solid to light yellow solid.

113694-64-7

32160000

solid

Protect from light and moisture.Very light-sensitive.

InChI=1S/C29H27N3O14.5K/c1-15-2-3-17(31(11-24(33)34)12-25(35)36)20(6-15)43-4-5-44-21-7-16-8-22(28-30-10-23(46-28)29(41)42)45-19(16)9-18(21)32(13-26(37)38)14-27(39)40;;;;;/h2-3,6-10H,4-5,11-14H2,1H3,(H,33,34)(H,35,36)(H,37,38)(H,39,40)(H,41,42);;;;;/q;5*+1/p-5

HQKWBBCTCLUGSX-UHFFFAOYSA-I

Chemical. CAS: 113694-64-7. Formula: C29H22N3O14 . 5K. MW: 831.99. Fura-2 pentapotassium salt is a widely used UV-excitable fluorescent calcium indicator. It is a fluorophore chelating probe for Ca2+ used widely in biological systems to analyze intracellular calcium levels. It can be used to detect calcium in cells. A diagnostic shift in the excitation spectrum from 363nm to 335nm indicates FURA-2 chelation to Ca2+. Upon calcium binding, the fluorescent excitation maximum of the indicator undergoes a blue shift from 363 nm (Ca2+-free) to 335 nm (Ca2+-saturated), while the fluorescence emission maximum is relatively unchanged at ~510 nm. Analysis of Ca2+ flux in and out of cells with Fura-2 introduces a powerful spectrographic handle to investigate and elucidate correlated biological events such as neuronal ion channel activation. A major use of Fura-2 is in drug discovery, for measuring intracellular calcium mobilization after activation of G protein-coupled receptors (GPCRs) and calcium channels. It can also be used as a zinc indicator or measuring membrane potential. Fura-2 fluorescence can be detected using flow cytometry, fluorescence microscopy, or certain fluoresence microplate readers. Fura-2 potassium salt is a cell-impermeable fluorescent probe for detection Ca2+ in a ratiometric mode. However, it may be loaded into cells via microinjection or scrape loading. Spectral data: lambdaEx 363nm, lambdaEm 512nm in water.

MFCD00083328

C29H22N3O14 . 5K

831.99

Vial

Fura-2 pentapotassium salt is a widely used UV-excitable fluorescent calcium indicator. It is a fluorophore chelating probe for Ca2+ used widely in biological systems to analyze intracellular calcium levels. It can be used to detect calcium in cells. A diagnostic shift in the excitation spectrum from 363nm to 335nm indicates FURA-2 chelation to Ca2+. Upon calcium binding, the fluorescent excitation maximum of the indicator undergoes a blue shift from 363 nm (Ca2+-free) to 335 nm (Ca2+-saturated), while the fluorescence emission maximum is relatively unchanged at ~510 nm. Analysis of Ca2+ flux in and out of cells with Fura-2 introduces a powerful spectrographic handle to investigate and elucidate correlated biological events such as neuronal ion channel activation. A major use of Fura-2 is in drug discovery, for measuring intracellular calcium mobilization after activation of G protein-coupled receptors (GPCRs) and calcium channels. It can also be used as a zinc indicator or measuring membrane potential. Fura-2 fluorescence can be detected using flow cytometry, fluorescence microscopy, or certain fluoresence microplate readers. Fura-2 potassium salt is a cell-impermeable fluorescent probe for detection Ca2+ in a ratiometric mode. However, it may be loaded into cells via microinjection or scrape loading. Spectral data: lambdaEx 363nm, lambdaEm 512nm in water.

>90% (HPLC)

[O-]C(C1=CN=C(O1)C2=CC3=C(O2)C=C(N(CC([O-])=O)CC([O-])=O)C(OCCOC4=C(N(CC([O-])=O)CC([O-])=O)C=CC(C)=C4)=C3)=O.[K+].[K+].[K+].[K+].[K+]

Soluble in water or DMSO.

Synthetic

Non-hazardous

Fluorescent Reagents

41105331

Stable for at least 2 years after receipt when stored at -20°C.