PBFI-AM
| Code | Size | Price |
|---|
| CDX-P0068-M001 | 1 mg | £462.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
AMBIENT
Storage:
-20°C
Documents
Further Information
Alternate Names/Synonyms:
Potassium indicator PBFI-AM; Potassium-binding benzofuran isophthalate-AM; PBFI diacetate
Appearance:
Orange paste.
CAS:
124549-23-1
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Protect from light and moisture.Very light-sensitive.
InChi:
InChI=1S/C58H62N2O24/c1-35(61)75-31-79-55(65)39-7-9-43(45(23-39)57(67)81-33-77-37(3)63)51-25-41-27-53(69-5)47(29-49(41)83-51)59-11-15-71-19-21-73-17-13-60(14-18-74-22-20-72-16-12-59)48-30-50-42(28-54(48)70-6)26-52(84-50)44-10-8-40(56(66)80-32-76-36(2)62)24-46(44)58(68)82-34-78-38(4)64/h7-10,23-30H,11-22,31-34H2,1-6H3
InChiKey:
AUKRBWNSOWVABU-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 124549-23-1. Formula: C58H62N2O24. MW: 1171.11. PBFI AM is a cell-permeable, potassium-sensitive fluorophore used to measure potassium changes in cells and intracellular compartments. As PBFI AM enters cells, it is hydrolyzed by intracellular esterases to produce PBFI. The affinity of PBFI for potassium is sodium-dependent (Kd=44 and 5.1mM with or without sodium, respectively). Spectral data: lambdaEx=370nm; lambdaEm=540nm in methanol.
MDL:
MFCD00083475
Molecular Formula:
C58H62N2O24
Molecular Weight:
1171.11
Package Type:
Vial
Product Description:
PBFI AM is a cell-permeable, potassium-sensitive fluorophore used to measure potassium changes in cells and intracellular compartments. As PBFI AM enters cells, it is hydrolyzed by intracellular esterases to produce PBFI. The affinity of PBFI for potassium is sodium-dependent (Kd=44 and 5.1mM with or without sodium, respectively). Spectral data: lambdaEx=370nm; lambdaEm=540nm in methanol.
Purity:
>98% (HPCE)
SMILES:
O=C(OCOC(C)=O)C(C=C1)=CC(C(OCOC(C)=O)=O)=C1C2=CC3=CC(OC)=C(N4CCOCCOCCN(C5=C(OC)C=C6C(OC(C7=C(C(OCOC(C)=O)=O)C=C(C(OCOC(C)=O)=O)C=C7)=C6)=C5)CCOCCOCC4)C=C3O2
Solubility Chemicals:
Soluble in DMSO or methanol.
Source / Host:
Synthetic
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at -20°C.
References
(1) P. Jezek, et al.; J. Biol. Chem. 265, 10522 (1990) | (2) S. Lindberg; Planta 195, 525 (1995) | (3) C.D. Bortner, et al.; J. Biol. Chem. 272, 32436 (1997) | (4) S.J. Halperin & J.P. Lynch; J. Exp. Bot. 54, 2035 (2003) | (5) R.W. Sabnis; Handbook of biological dyes and stains (2010)