Gram's fuchsin Solution

Fuchsin solution; Basic Red 9; Magenta O; Basic Violet 14

Dark red liquid.

632-99-5

32160000

liquid

GHS08

Protect from light and moisture.

H350

InChI=1S/C20H19N3.ClH/c1-13-12-16(6-11-19(13)23)20(14-2-7-17(21)8-3-14)15-4-9-18(22)10-5-15;/h2-12,21H,22-23H2,1H3;1H/b20-14-,21-17?;

NIKFYOSELWJIOF-SVFFXJIWSA-N

Chemical. CAS: 632-99-5. Formula: C20H20ClN3. MW: 337.85. Gram's Fuchsin Solution is a reagent that could be used in qualitative procedures to differentiate gram-negative from gram-positive organisms. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution, a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue/violet. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized. Gram-negative bacteria will be counterstained by Gram's Fuchsin solution and appear red/pink. Both Gram-positive bacteria and Gram-negative bacteria pick up the counterstain. The counterstain, however, is unseen on Gram-positive bacteria because of the darker crystal violet stain.

MFCD18071399

C20H20ClN3

337.85

Vial

P201-P308 + P313

Gram's Fuchsin Solution is a reagent that could be used in qualitative procedures to differentiate gram-negative from gram-positive organisms. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution, a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue/violet. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized. Gram-negative bacteria will be counterstained by Gram's Fuchsin solution and appear red/pink. Both Gram-positive bacteria and Gram-negative bacteria pick up the counterstain. The counterstain, however, is unseen on Gram-positive bacteria because of the darker crystal violet stain.

NC1=CC=C(/C(C2=CC(C)=C(N)C=C2)=C3C=CC(C=C/3)=N)C=C1.Cl

Soluble in water.

Synthetic

Non-hazardous

Fluorescent Reagents

41105331

Stable for at least 1 year after receipt when stored at +4°C.