6-Carboxy-2',7'-dichlorofluorescein DA
| Code | Size | Price |
|---|
| CDX-C0334-M100 | 100 mg | £230.00 |
Quantity:
| CDX-C0334-M250 | 250 mg | £450.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
AMBIENT
Storage:
Short Term: +4°C, Long Term: -20°C
Documents
Further Information
Alternate Names/Synonyms:
6-CDCF DA; 6-Carboxy-DCF DA
Appearance:
White to off-white powder.
CAS:
144489-10-1
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Protect from light and moisture.
InChi:
InChI=1S/C25H14Cl2O9/c1-10(28)33-21-8-19-15(6-17(21)26)25(14-5-12(23(30)31)3-4-13(14)24(32)36-25)16-7-18(27)22(34-11(2)29)9-20(16)35-19/h3-9H,1-2H3,(H,30,31)
InChiKey:
FCNONRRKVDBQRD-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 144489-10-1. Formula: C25H14Cl2O9. MW: 529.29. Fluorescent indicator. The non-fluorescent 6-CDCF DA passively diffuses across the cell membrane and is hydrolyzed to fluorescent CDCF by intracellular esterases. It has been shown to be highly sensitive, long-lasting, cell-permeant and fluorescein-based tracer for quantifying ROS generation. Substrate for MRP2 and MRP3 and therefore the function of the efflux transporters (hepatic exrection) and multi-drug resistance associated proteins MRP2 and MRP3 can be measured using 6-CDCF DA. The CDCFDA/CDCF technoique provides a fast screening tool for MRP2 substrates and/or inhibitors. Spectral data: lambdaEx=502nm; lambdaEm=530nm.
MDL:
MFCD00134867
Molecular Formula:
C25H14CI2O9
Molecular Weight:
529.29
Package Type:
Vial
Product Description:
Fluorescent indicator. The non-fluorescent 6-CDCF DA passively diffuses across the cell membrane and is hydrolyzed to fluorescent CDCF by intracellular esterases. It has been shown to be highly sensitive, long-lasting, cell-permeant and fluorescein-based tracer for quantifying ROS generation. Substrate for MRP2 and MRP3 and therefore the function of the efflux transporters (hepatic exrection) and multi-drug resistance associated proteins MRP2 and MRP3 can be measured using 6-CDCF DA. The CDCFDA/CDCF technoique provides a fast screening tool for MRP2 substrates and/or inhibitors. Spectral data: lambdaEx=502nm; lambdaEm=530nm.
Purity:
>97% (NMR)
SMILES:
O=C(O1)C2=C(C=C(C(O)=O)C=C2)C31C4=C(C=C(OC(C)=O)C(Cl)=C4)OC5=CC(OC(C)=O)=C(Cl)C=C53
Solubility Chemicals:
Soluble in DMSO or chloroform. Insoluble in water.
Source / Host:
Synthetic
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at -20°C.
References
(1) J.P. Kehrer & T. Paraidathathu; Free Rad. Res. Commun. 16, 217 (1992) | (2) M.J. Zamek-Gliszczynski, et al.; J. Pharmacol. Exp. Ther. 304, 801 (2003) | (3) S. Siissalo, et al.; Eur. J. Pharm. Biopharm. 71, 332 (2009) | (4) A.I. Pogue, et al.; Int. J. Mol. Sci. 13, 9615 (2012) | (5) J.A.Sinclair, et al.; Toxicol. In Vitro 27, 451 (2013)