Biocytine
| Code | Size | Price |
|---|
| CDX-B0412-M100 | 100 mg | £121.00 |
Quantity:
| CDX-B0412-M250 | 250 mg | £255.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
AMBIENT
Storage:
-20°C
Documents
Further Information
Alternate Names/Synonyms:
Bct; Nepsilon-(+)-Biotinyl-L-lysine; epsilon-N-(d-Biotinyl)-L-lysine;
Appearance:
White to off-white powder.
CAS:
576-19-2
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Protect from light and moisture.
InChi:
InChI=1S/C16H28N4O4S/c17-10(15(22)23)5-3-4-8-18-13(21)7-2-1-6-12-14-11(9-25-12)19-16(24)20-14/h10-12,14H,1-9,17H2,(H,18,21)(H,22,23)(H2,19,20,24)/t10?,11-,12-,14-/m0/s1
InChiKey:
BAQMYDQNMFBZNA-WBGPXRNDSA-N
Long Description:
Chemical. CAS: 576-19-2. Formula: C16H28N4O4S. MW: 372.48. Biocytin is a conjugate of biotin and lysine known formally as epsilon-N-(d-biotinyl)-L-lysine. The addition of lysine to biotin increases the chain length extending from biotin, allowing the synthesis of medium-chain reagents. It also provides terminal carboxyl and amino groups for derivatization or conjugation to proteins and other molecules. Covalent linking reactions typically involve carbodiimide or NHS-ester crosslinking chemistries. Biocytin is also used as an anterograde, retrograde, or intracellular neuroanatomical tracer that is fixable with aldehyde-based fixatives. It is also used in biotinidase assays. Biocytin may be injected into brain by iontophoresis or by pressure injection methods, and localized in tissue sections using avidin-conjugated labels.
MDL:
MFCD00077319
Molecular Formula:
C16H28N4O4S
Molecular Weight:
372.48
Package Type:
Vial
Product Description:
Biocytin is a conjugate of biotin and lysine known formally as epsilon-N-(d-biotinyl)-L-lysine. The addition of lysine to biotin increases the chain length extending from biotin, allowing the synthesis of medium-chain reagents. It also provides terminal carboxyl and amino groups for derivatization or conjugation to proteins and other molecules. Covalent linking reactions typically involve carbodiimide or NHS-ester crosslinking chemistries. Biocytin is also used as an anterograde, retrograde, or intracellular neuroanatomical tracer that is fixable with aldehyde-based fixatives. It is also used in biotinidase assays. Biocytin may be injected into brain by iontophoresis or by pressure injection methods, and localized in tissue sections using avidin-conjugated labels.
Purity:
>95% (NMR)
SMILES:
[H][C@]12CS[C@@H](CCCCC(NCCCCC(N)C(O)=O)=O)[C@@]1([H])NC(N2)=O
Solubility Chemicals:
Soluble in PBS or water (20 mg/ml). We do not recommend to store aqueous solutions for more than one day.
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at -20°C.
References
(1) M. Bodanszky & D.T. Fagan; JACS 99, 235 (1977) | (2) E. Friauf, et al.; J. Neurosci. 10, 2601 (1990) | (3) X.J. Sun, et al.; J. Histochem. Cytochem. 46, 263 (1998) | (4) A. Erisir & C. Aoki; J. Neurosci. Methods 81, 189 (1998) | (5) C. Koebbert, et al; Prog. Neurobiol. 62, 327 (2000) | (6) S.L. Chang, et al.; J. Neurosci. Methods 97, 1 (2000) | (7) K. Kumasaka, et al.; Clin. Chim. Acta 306, 71 (2001) | (8) Y. Morozov, et al.; J. Neurosci. Methods 117, 81 (2002) | (9) G. Roth, et al.; J. Comp. Neurol. 478, 35 (2004) | (10) H.G. Xue, et al.; Brain Res. Brain Res. Protoc. 13, 106 (2004) | (11) M.A. MacNeil & P.A. Gaul; J. Comp. Neurol. 506, 6 (2008) | (12) R. Bopp, et al.; PLoS Biol. 12, 1 (2014)