Mouse anti Mouse p27

Nordic MuBio
Product Code: EXA-C155M
Product Group: Primary Antibodies
Supplier: Nordic MuBio
CodeSizePrice
EXA-C155M100 ug£316.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: DCS72
Regulatory Status: RUO
Target Species:
  • Canine (dog)
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
Ship at ambient temperature
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Further Information

Applications Description:
Western Blot (5-10 ?g/ml), Immunohistochemistry, Immunoprecipitation (co-precipitates cdk4)
Background:
The p27 kip1 functions as a negative regulator of G1 mouse expression and has been proposed to function as a possible mediator of TGF induced G1 arrest.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Cell Cycle
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Product:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Product Form:
Unconjugated
Product Stability:
See expiration date on vial
Purification Method:
Ammonium Sulfate Precipitation
Source:
The immunogen for the anti-mouse p27 was a his-tagged recombinant full length mouse p27 protein recognizing a 27kDa protein.
Synonyms:
p27Kip1; Cyclin-dependent kinase inhibitor p27; Cyclin-dependent kinase inhibitor 1B
UniProt:
P46527 (Human) P46414 (Mouse) O08769 (Rat) Q6SLL5 (Dog)

References

1) K. Polyak, et.al. (1994) Gene & Dev.8, 9-22