Mouse anti Human Bence Jones kappa (surface and hidden determinants)
| Code | Size | Price |
|---|
| MAHu_BJK | 0.5 mg | £375.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: NI 285 NI 250
Regulatory Status: RUO
Storage:
The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4°C not refrozen and preferably used the same day.
Further Information
Applications Description:
Indirect immunofluorescence,ELISA.
Background:
The reactivity of this preparation of two monoclonal antibodies is restricted to a surface determinant on the kappa light chain. It is reacting with polyclonal and monoclonal immunoglobulins of the kappa type, as well as free kappa light chains as tested in direct binding enzyme immunoassay, immunoblotting,
immunoprecipitation and direct immunoperoxidase staining. To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in indirect techniques as ELISA, indirect immunoperoxidase staining and immunoblotting. The optimum working dilution is an assayrelated characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Formulation:
Delipidated, heat inactivated, lyophilized, stable whole ascites. No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration 1.0 mg/ml. No foreign proteins added.
Product:
Delipidated, heat inactivated, lyophilized, stable whole ascites. No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration 1.0 mg/ml. No foreign proteins added. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water.
Source:
Highly purified Bence Jones kappa proteins isolated from pooled human urine.
Specificity:
Monoclonal Mouse antiSerum to Human Ig kappa chain, free and bound