Mouse anti Human IgE (class specific) conjugated with Horseradish peroxidase

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Nordic MuBio
Product Code: MAHu_IgE_PO
Product Group: Secondary Antibody
Supplier: Nordic MuBio
CodeSizePrice
MAHu_IgE_PO0.2 mg£573.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: NI 307
Regulatory Status: RUO
Target Species: Human
Applications:
  • Dot Blot
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunoblotting
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
Storage:
The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4°C not refrozen and preferably used the same day.

Further Information

Applications Description:
ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
Background:
The reactivity of the this monoclonal antibody is restricted to polyclonal and monoclonal IgE, as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic immunoglobulins. To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:250; in ELISA from 1:250 upwards; in Western blotting from 1:500 upwards.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Label:
HRP
Product:
Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 0.4 mg/ml. Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. No foreign proteins added. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water.
Product Form:
HRP
Source:
Highly purified monoclonal IgE isolated from human serum.
Specificity:
Horseradish peroxidase conjugated purified monoclonal Mouse antibody to Human IgE, isotype specific.