Mouse anti Human IgE (class specific)
| Code | Size | Price |
|---|
| MAHu_IgE | 0.5 mg | £375.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: NI 307
Regulatory Status: RUO
Storage:
The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4°C not refrozen and preferably used the same day.
Further Information
Applications Description:
Indirect immunofluorescence,ELISA.
Background:
The reactivity of the antiserum is restricted to the IgE molecule as tested in ELISA, immunoblotting, immunoprecipitation. Precipitation takes place under special test conditions in the presence of polyethylene glycol. To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgE and immunoblotting. The optimum working dilution is an assayrelated characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100; in ELISA from 1:50 upwards; in Western blotting from 1:100 upwards. These data should be interpreted as general recommendations only.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Formulation:
Delipidated, heat inactivated, lyophilized, stable whole ascites IgG concentration is 1 mg/ml. No foreign proteins added.
Product:
Delipidated, heat inactivated, lyophilized, stable whole ascites IgG concentration is 1 mg/ml. No foreign proteins added. Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water.
Source:
Highly purified monoclonal IgE isolated from human serum
Specificity:
Monoclonal Mouse antibody to Human IgE, isotype specific