Mouse anti Human IgA1+IgA2

Nordic MuBio
Product Code: MAHu_IgAc_MAb
Product Group: Secondary Antibody
Supplier: Nordic MuBio
CodeSizePrice
MAHu_IgAc_MAb0.2 mg£463.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: NI 69 (A89-034) and NI 184 (A89-035)
Regulatory Status: RUO
Storage:
The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4°C not refrozen and preferably used the same day

Further Information

Applications Description:
Indirect immunofluorescence,ELISA.
Background:
The reactivity of the antiserum is restricted to determinants on the CH2 domain of IgA. It reacts with both subclasses of IgA as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). To identify the presence of IgA in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgA, haemagglutination and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:200 upwards; in Western blotting from 1:500 upwards. These data should be interpreted as general recommendations only.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Formulation:
Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2) IgG concentration is 0.4mg/ml. No foreign proteins added.
Product:
Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2) IgG concentration is 0.4mg/ml. No foreign proteins added. Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water.
Source:
Highly purified monoclonal IgA isolated from human serum.
Specificity:
Purified monoclonal Mouse antibody to Human IgA, isotype specific