Mouse anti Human IgA1 (subclass specific)

Nordic MuBio
Product Code: MAHu_IgA1
Product Group: Secondary Antibody
Supplier: Nordic MuBio
CodeSizePrice
MAHu_IgA10.5 mg£375.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: NI 69-11 (A89-036)
Regulatory Status: RUO
Storage:
The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4°C not refrozen and preferably used the same day.

Further Information

Applications Description:
Indirect immunofluorescence,ELISA.
Background:
The reactivity of the antiserum is restricted to a subclass specific determinant on the CH2 domain of IgA1 as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). To identify the presence of IgA1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgA1, haemagglutination and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:3000 to 1:50000. These data should be interpreted as general recommendations only.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Formulation:
Delipidated, heat inactivated, lyophilized, stable whole ascites IgG concentration 1 mg/ml. No foreign proteins added.
Product:
Delipidated, heat inactivated, lyophilized, stable whole ascites IgG concentration 1 mg/ml. No foreign proteins added. Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water.
Source:
Highly purified monoclonal IgA1 isolated from pooled human serum
Specificity:
Monoclonal Mouse antiSerum to Human IgA1, subclass specific.