Recombinant rabbit anti vimentin
| Code | Size | Price |
|---|
| MUB1904P | 0.1 mg | £679.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Antibody Clone: RV205
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Immunoblotting
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
Shipping:
At ambient temperature
Storage:
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use an aliquot is thawed slowly in the dark at ambient temperature spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C not refrozen and preferably used the same day. If a slight precipitation occurs upon storage this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Documents
Further Information
Applications Description:
MUB1904P (clone RV205) is suitable for immunoblotting, immunocytochemistry, and immunohistochemistry on frozen and paraffin-embedd tissue sections. Optimal antibody dilution should be determined by titration; recommended range is 1:100 ? 1:200 for immunohistochemistry with fluorochrome conjugated secondary antibodies or with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 ? 1:1000 for immunoblotting applications.
Background:
Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water.
This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water.
This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Field of Interest:
Cancer|Cardiovascular|Cytoskeleton|Immunology|Neurobiology|Stem cell research
Product:
Each vial contains 100 ul 1 mg/ml ProtA purified recombinant monoclonal antibody in PBS containing 0.09% sodium azide.
The purity of the antibody is >98% as determined by SDS-Polyacrylamide gel electrophoresis.
The endotoxin level is < 1EU/mg as determined by the LAL chromogenic endotoxin assay.
The purity of the antibody is >98% as determined by SDS-Polyacrylamide gel electrophoresis.
The endotoxin level is < 1EU/mg as determined by the LAL chromogenic endotoxin assay.
Source:
MUB1904P (clone RV205) is a recombinant rabbit anti vimentin monoclonal antibody produced in mammalian HEK293 cells.
Clone RV205 is a reformatted antibody (species switch), produced by cloning the antibody sequence of the mouse anti vimentin hybridoma RV202 (MUB1900P), which was derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.
Clone RV205 is a reformatted antibody (species switch), produced by cloning the antibody sequence of the mouse anti vimentin hybridoma RV202 (MUB1900P), which was derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.
Species Reactivity:
MUB1904P (clone RV205) reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.
When applied to either frozen or paraffin sections of human tissues the antibody reacted with the appropriate tissues and cells, such as endothelia, vessels, stellate cells, glomerular podocytes, nerves, vessels, myoepithelial cells, adipocytes, endometrial glands, and Sertoli cells, with no staining in epithelial cells.
When applied to either frozen or paraffin sections of human tissues the antibody reacted with the appropriate tissues and cells, such as endothelia, vessels, stellate cells, glomerular podocytes, nerves, vessels, myoepithelial cells, adipocytes, endometrial glands, and Sertoli cells, with no staining in epithelial cells.
Specificity:
Human and mouse vimentin
UniProt:
P08670