Goat anti Monkey IgG IgA IgM (heavy and light chains)

Nordic MuBio
Product Code: GAMon_Ig_7S
Product Group: Secondary Antibody
Supplier: Nordic MuBio
CodeSizePrice
GAMon_Ig_7S10 mg£399.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Storage:
The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use an aliquot is thawed slowly at ambient temperature spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C not refrozen and preferably used the same day. If a slight precipitation occurs upon storage this should be removed by centrifugation. It will not affect the performance of the product.

Further Information

Applications Description:
Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.
Background:
The reactivity of the antiserum is directed to the major isotypes of the monkey immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal monkey plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained. This IgG fraction allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Formulation:
Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
Product:
Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.
Source:
Highly purified IgG and pools of homogenous IgA and IgM isolated from rhesus monkey serum. Freund?s complete adjuvant is used in the first step of the immunization procedure.
Specificity:
Purified IgG fraction of polyclonal Goat antiSerum to Monkey immunoglobulins IgG, IgA, IgM, heavy and light chains.