Rabbit anti Rat IgG1 IgG2a IgG2b IgG2c IgA IgM (heavy and light chains) conjugated with FITC
| Code | Size | Price |
|---|
| RARa_Ig_FITC | 1 ml | £463.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Storage:
The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use an aliquot is thawed slowly in the dark at ambient temperature spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C not refrozen and preferably used the same day. If a slight precipitation occurs upon storage this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Packing Vial with 1 ml lyophilized
Further Information
Applications Description:
ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
Background:
The reactivity of the antiserum is directed to all known isotypes of the rat immunoglobulin system (classes, subclasses and light chain types) including antibodies to common determinants, to class and subclass specific determinants, and to the surface determinants of the common Fab portion as tested by immunoelectrophoresis and double radial immunodiffusion. It does not react with any non-Ig protein in rat serum. To identify rat immunoglobulins, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of rat origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Formulation:
FITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added
Product:
FITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.
Product Form:
FITC
Source:
Purified polyclonal rat IgG, including all subclasses, and pools of homogenous immunoglobulins. Freund?s complete adjuvant is used in the first step of the immunization procedure.
Specificity:
Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Rabbit antiSerum to Rat immunoglobulins, heavy and light chains.