Rabbit anti Mouse IgM (Fc specific)
| Code | Size | Price |
|---|
| RAM_IgM(Fc)_7S | 10 mg | £399.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Storage:
The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use an aliquot is thawed slowly at ambient temperature spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C not refrozen and preferably used the same day. If a slight precipitation occurs upon storage this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Further Information
Applications Description:
Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.
Background:
The defined antibody specificity is restricted to the Fc part of the IgM molecule, as tested against mouse sera at the level of sensitivity of direct immunofluorescence staining methods applied to lymphoid cells with the FITC conjugated form of this product. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations, against mouse serum a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with the purified IgM. It does not react with IgG, IgG/Fab fragments and IgA. As unlabelled primary or secondary antibody reagent for indirect detection of IgM in mouse cells, tissues and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting. When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling techniques, the optimum concentration of this product should be established before.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Formulation:
Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
Product:
Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.
Source:
Highly purified homogenous IgM isolated from mouse serum. Immunization with intact (19S) and split IgM (7S). Freund?s complete adjuvant is used in the first step of the immunization procedure.
Specificity:
Purified IgG fraction of polyclonal Rabbit antiSerum to Mouse IgM, Fc specific