Sheep anti Mouse Ig lambda light chains (free and bound)
| Code | Size | Price |
|---|
| ShAM_BJL(SD+HD) | 1 ml | £285.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Sheep
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Storage:
The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C not refrozen and preferably used the same day. If a slight precipitation occurs upon storage this should be removed by centrifugation. It will not affect the performance of the product.
Further Information
Applications Description:
Precipitation assays. In immunoelectrophoresis use 2 ul serum or equivalent against 120 ul antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ul antiserum in 3 mm diameter center well and 2 ul serum samples (neat and serially diluted in 2 mm diameter peripheral wells. When carrying out analyses of Bence Jones proteins or free light chains in urine or serum using an immunodiffusion technique, special attention should be given to their faster rate of diffusion as compared to that of the antibody molecules in the antiserum. If they both start to diffuse into the gel in a double radial diffusion at the same time, an excess of the antigen may easily prevent visible precipitation. To prevent false negative results, the antiserum should be diffuse for about 2 hours before the antigen reservoir is filled.
Background:
The reactivity of the antiserum is directed to the surface and hidden determinants of Ig lambda light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the lambda type, Bence Jones proteins as well as free light chains of the lambda type. This antiserum does not react with any other protein of mouse serum or plasma. To detect and identify the light chain type of immunoglobulins or free light chains in serum or other body fluids by immunoelectrophoresis or double radial immuno- diffusion.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Formulation:
Delipidated, heat inactivated lyophilized whole serum. No preservative added, as it may interfere with the antibody activity.
Product:
Delipidated, heat inactivated lyophilized whole serum. No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles.
Source:
A pool of purified Bence Jones lambda proteins isolated from mouse urine. Freund?s complete adjuvant is used in the first step of the immunization procedure.
Specificity:
Precipitating polyclonal Sheep antiSerum to free and bound Mouse Ig lambda light chain.