Swine anti Mouse IgG (heavy and light chains)

Precipitation assays. In immunoelectrophoresis use 2 ul or equivalent against 120 ul antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ul antiserum in a 3 mm diameter centre well and 2 ul serum samples (neat and diluted) in 2 mm diameter peripheral wells.

The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in mouse serum, as tested by immunoelectrophoresis. Potential uses: In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in mouse serum; as bridging reagent in a peroxidase antiperoxidase technique using M/PAP; to prepare an immunoadsorbent for the production of Ig-free mouse serum, plasma or non-Ig preparations.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Neat liquid antiserum containing 0.05% sodium azide as a preservative.

Neat liquid antiserum containing 0.05% sodium azide as a preservative.

Purified normal IgG isolated from pooled mouse serum. Freund?s complete adjuvant is used in the first step of the immunization procedure.

Precipitating polyclonal swine antiserum to mouse IgG, heavy and light chains.