Swine anti Human IgM (Fc specific) conjugated with FITC
| Code | Size | Price |
|---|
| SwAHu_IgM(Fc)_FITC | 1 ml | £242.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Porcine (Pig)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Storage:
The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use an aliquot is thawed slowly in the dark at ambient temperature spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C not refrozen and preferably used the same day. If a slight precipitation occurs upon storage this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Further Information
Applications Description:
ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
Background:
The reactivity of the antiserum is directed to the Fc subunits of the IgM molecule which expresses strict isotypic specificity. The conjugate does not react with any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. To identify IgM at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Formulation:
FITC-coupled purified hyperimmune swine IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
Product:
FITC-coupled purified hyperimmune swine IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.
Product Form:
FITC
Source:
Purified normal IgM isolated from pooled human serum. Freund?s complete adjuvant is used in the first step of the immunization procedure.
Specificity:
Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Swine antiSerum to Human IgM, Fc specific.