Ubiquitin Activating Enzyme E1 [UBA1] (human) (rec.) (His)

Ubiquitin E1 Enzyme; Ubiquitin-like Modifier-activating Enzyme 1; A1S9T; AMCX1; GXP1; POC20; SMAX2

Lot dependent.

32160000

liquid

Liquid. In 50mM HEPES pH 7.5, 150mM sodium chloride, 10% glycerol, 2mM TCEP.

Aliquot to avoid freeze/thaw cycles.

Protein. Human UBA1 (aa12-1058) is fused at the N-terminus to a His-tag. Source: Sf21 cells. Liquid. In 50mM HEPES pH 7.5, 150mM sodium chloride, 10% glycerol, 2mM TCEP. Purity: >95% (SDS-PAGE). UBA1, the canonical ubiquitin E1 activating enzyme is a 118kDa protein, which forms a homodimer in its active state and catalyzes the first step of the ubiquitin conjugation cascade. It activates ubiquitin in an ATP-dependent mechanism where ATP is hydrolyzed to AMP and PPi, a ubiquitin C-terminal adenylate intermediate is formed, then transferred to the E1's active site cysteine through a thioester bond. This thioester is then transferable to an E2 conjugating enzyme's active site cysteine.

~120kDa

P22314

Plastic Vial

UBA1, the canonical ubiquitin E1 activating enzyme is a 118kDa protein, which forms a homodimer in its active state and catalyzes the first step of the ubiquitin conjugation cascade. It activates ubiquitin in an ATP-dependent mechanism where ATP is hydrolyzed to AMP and PPi, a ubiquitin C-terminal adenylate intermediate is formed, then transferred to the E1's active site cysteine through a thioester bond. This thioester is then transferable to an E2 conjugating enzyme's active site cysteine.

>95% (SDS-PAGE)

Human UBA1 (aa12-1058) (Accession Nr. P22314 http://www.ncbi.nlm.nih.gov/protein/P22314 ) is fused at the N-terminus to a His-tag.

Sf21 cells

His

Non-hazardous

Other Proteins

12352202

Stable for at least 1 year after receipt when stored at -80°C.