CD32 Antibody / FCGR2A

Functional studies (order BSA/sodium azide-free format),Immunofluorescence: 0.5-1ug/ml

Optimal dilution of the CD32 antibody should be determined by the researcher.

This mAb reacts with a CD32 (FcgRII) epitope distinct from that defined by mAb 8.26 and the epitope overlaps with that of mAb 7.30 (cluster 4). It displays a stronger reaction with Daudi than with U937 cells. The epitope is located in domain 2 of FcgRIIa. Its Fab'2 fragments block immune complex binding. CD32 (FcgRII) is a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

K562 and FcgRII+L cells were used as the immunogen for the CD32 antibody.

This CD32 antibody is available for research use only.

Cytoplasm, plasma membrane

Protein G affinity chromatography

Human

P12318