HA Tag Antibody

NSJ Bioreagents
Product Code: NSJ-V8290
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8290-20UG20 ug£264.00
Quantity:
NSJ-V8290-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 16.43
Regulatory Status: RUO
Application: Western Blot (WB)
Storage:
Store the HA Tag antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Immunofluorescent staining of PFA-fixed human MCF7 cells that have been transfected with an HA-tagged protein, with HA Tag antibody (clone 16.43, green) and Reddot nuclear stain (red).~
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Western blot testing of HA-tagged recombinant prote

Immunofluorescent staining of PFA-fixed human MCF7 cells that have been transfected with an HA-tagged protein, with HA Tag antibody (clone 16.43, green) and Reddot nuclear stain (red).~
Western blot testing of HA-tagged recombinant prote

Further Information

Application Details :
ELISA (order BSA-free format for coating),Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate),Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Amino acids YPYDVPDYA were used as the immunogen for this HA Tag antibody.
Limitation:
This HA Tag antibody is available for research use only.
Purity:
Protein G affinity chromatography
Species Reactivity :
HA tag