CD1b Antibody

Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Functional studies (order BSA/sodium azide-free format)

Optimal dilution of the CD1b antibody should be determined by the researcher.

The mouse monoclonal antibody recognizes CD1b, a 44kDa type I glycoprotein associated with beta2-microglobulin (Workshop IV; Code T015). It is expressed on dendritic cells, Langerhans cells, thymocytes, and T acute lymphoblastic leukemia cells. The CD1 multigene family encodes five forms of the CD1 T-cell surface glycoprotein in human, designated CD1A, 1B, 1C, 1D and 1E. CD1, a type 1 membrane protein, has structural similarity to the MHC class I antigen and has been shown to present lipid antigens for recognition by T lymphocytes. Constitutive endocytosis of CD1B molecules and the differential sorting of MHC class II from lysosomes separate peptide- and lipid antigen-presenting molecules during dendritic cell maturation. CD1B is also expressed in interdigitating cells.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Stimulated human leukocytes were used as the immunogen for the CD1b antibody.

This CD1b antibody is available for research use only.

Cell surface, cytoplasmic

PEG precipitation

Human

P29016