NSJ Bioreagents

HLA-A Antibody

Product Code:
 
NSJ-V2571
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
108-2C5
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
 
Store the HLA-A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
Flow cytometry testing of human Raji cells with HLA-A antibody (clone 108-2C5); Red=isotype control, Blue= HLA-A antibody.~
2 / 2
SDS-PAGE analysis of purified, BSA-free HLA-A antibody (clone 108-2C5) as confirmation of integrity and purity.
❮❯

Flow cytometry testing of human Raji cells with HLA-A antibody (clone 108-2C5); Red=isotype control, Blue= HLA-A antibody.~
SDS-PAGE analysis of purified, BSA-free HLA-A antibody (clone 108-2C5) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V2571-20UG20 ug£264.00
Quantity:
NSJ-V2571-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the HLA-A antibody should be determined by the researcher.
Description:
HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation. This mAb is useful for HLA molecular typing of peripheral blood leukocytes as well as a large number of leukemic cell lines. It reacts with an intralocus determinant present on a limited number of HLA-A locus-encoded gene products (HLA-A2, -A3, -A28, -A29, -A30, -A31 and Aw33). Its epitope maps between aa65-to-aa80 of the alpha1 domain of the HLA-A. This mAb recognizes an intralocus determinant present on a limited number of HLA-A locus-encoded gene products (HLA-A2, -A3, A28, -A29, -A30, -A31 and -Aw33). Furthermore, by testing its reactivity with HLA-A2 natural variants and mutants, the importance of amino acid residues 79 and/or 80 of the alpha1 domain was demonstrated in the formation of an intralocus HLA-A determinant.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Normal human peripheral blood lymphocytes were used as the immunogen for the HLA-A antibody.
Limitation:
This HLA-A antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P30443