CYP2C19 Antibody / Cytochrome p450 2C19

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NSJ Bioreagents
Product Code: NSJ-RQ5862
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5862-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 10G5
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the CYP2C19 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
IHC staining of FFPE human liver cancer with CYP2C19 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
Western blot testing of 1) monkey liver, 2) rat liver and 3) mouse liver lysate with CYP2C19 antibody. Predicted molecular weight ~56 kDa.
3 / 3
Flow cytometry testing of human A431 cells with CYP2C19 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CYP2C19 antibody.
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IHC staining of FFPE human liver cancer with CYP2C19 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) monkey liver, 2) rat liver and 3) mouse liver lysate with CYP2C19 antibody. Predicted molecular weight ~56 kDa.
Flow cytometry testing of human A431 cells with CYP2C19 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CYP2C19 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the CYP2C19 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Cytochrome P450 2C19 (abbreviated CYP2C19) encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to metabolize many xenobiotics, including the anticonvulsive drug mephenytoin, omeprazole, diazepam and some barbiturates. Polymorphism within this gene is associated with variable ability to metabolize mephenytoin, known as the poor metabolizer and extensive metabolizer phenotypes. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids QNQQSEFTIENLVITAADLLGAGTETT from the human protein were used as the immunogen for the CYP2C19 antibody.
Limitation:
This CYP2C19 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P33261