PPT1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54333
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54333-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54333-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 1117CT11.2.1.4
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the PPT1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human cerebellum tissue with PPT1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of FFPE mouse cerebellum tissue with PPT1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 5
IHC testing of FFPE rat cerebellum tissue with PPT1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 5
Western blot testing of human HepG2 cell lysate with PPT1 antibody. Predicted molecular weight ~34 kDa.
5 / 5
Flow cytometry testing of fixed and permeabilized human HepG2 cells with PPT1 antibody; Blue=isotype control, Green= PPT1 antibody.
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IHC testing of FFPE human cerebellum tissue with PPT1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE mouse cerebellum tissue with PPT1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE rat cerebellum tissue with PPT1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human HepG2 cell lysate with PPT1 antibody. Predicted molecular weight ~34 kDa.
Flow cytometry testing of fixed and permeabilized human HepG2 cells with PPT1 antibody; Blue=isotype control, Green= PPT1 antibody.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Flow cytometry: 1:25 (1x10e6 cells),Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the PPT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Full length recombinant human protein was used as the immunogen for the PPT1 antibody.
Limitation:
This PPT1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity
Species Reactivity :
Human
Uniprot #:
P50897