Bcl10 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54525
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54525-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54525-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 1185CT13.2.1.2
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the BCL10 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human kidney tissue with BCL10 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
IHC testing of FFPE mouse kidney tissue with BCL10 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Western blot testing of human Daudi cell lysate with BCL10 antibody. Expected molecular weight: 26~33 kDa.
4 / 4
Flow cytometry testing of human HeLa cells with BCL10 antibody; Blue=isotype control, Green= BCL10 antibody.
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IHC testing of FFPE human kidney tissue with BCL10 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE mouse kidney tissue with BCL10 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human Daudi cell lysate with BCL10 antibody. Expected molecular weight: 26~33 kDa.
Flow cytometry testing of human HeLa cells with BCL10 antibody; Blue=isotype control, Green= BCL10 antibody.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Flow cytometry: 1:25 (1x10e6 cells), Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the BCL10 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the BCL10 antibody.
Limitation:
This BCL10 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Protein G affinity
Species Reactivity :
Human
Uniprot #:
O95999