DDX1 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ6287
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6287-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 11E5.
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the DDX1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 5
Flow cytometry testing of human MCF7 cells with DDX1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DDX1 antibody.
2 / 5
Western blot testing of human 1) K562, 2) Caco-2, 3) U-2 OS, 4) HEK293, 5) U-87 MG, 6) HeLa, 7) A549, 8) rat heart, 9) rat kidney, 10) rat skeletal muscle, 11) rat lung, 12) mouse heart, 13) mouse kidney and 14) mouse lung lysate with DDX1 antibody. Predicted molecular weight ~86 kDa.
3 / 5
IHC staining of FFPE human rectal cancer with DDX1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
IHC staining of FFPE human renal carcinoma with DDX1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 5
IHC staining of FFPE human pancreatic cancer with DDX1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Flow cytometry testing of human MCF7 cells with DDX1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DDX1 antibody.
Western blot testing of human 1) K562, 2) Caco-2, 3) U-2 OS, 4) HEK293, 5) U-87 MG, 6) HeLa, 7) A549, 8) rat heart, 9) rat kidney, 10) rat skeletal muscle, 11) rat lung, 12) mouse heart, 13) mouse kidney and 14) mouse lung lysate with DDX1 antibody. Predicted molecular weight ~86 kDa.
IHC staining of FFPE human rectal cancer with DDX1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal carcinoma with DDX1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human pancreatic cancer with DDX1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the DDX1 antibody should be determined by the researcher.
Description:
ATP-dependent RNA helicase DDX1 is an enzyme that in humans is encoded by the DDX1 gene. It is mapped to 2p24.3. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids K562-F740) was used as the immunogen for the DDX1 antibody.
Limitation:
This DDX1 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q92499