GATA3 Antibody

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NSJ Bioreagents
Product Code: NSJ-F52445
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F52445-0.08ML0.08 ml£260.00
Quantity:
NSJ-F52445-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b, k
Antibody Clonality: Monoclonal
Antibody Clone: 1259CT707.136.102
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the GATA3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Western blot analysis of lysate from (1) CEM, (2) HL-60 and (3) SH-SY5Y cell lines using GATA3 antibody at 1:1000. Predicted molecular weight: 50 kDa
2 / 3
Fluorescent image of MCF-7 cells stained with GATA3 antibody at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
3 / 3
Flow cytometric analysis of MCF-7 cells using GATA3 antibody (green) and an isotype control of mouse IgG2b (blue); Ab was diluted at 1:25.
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Western blot analysis of lysate from (1) CEM, (2) HL-60 and (3) SH-SY5Y cell lines using GATA3 antibody at 1:1000. Predicted molecular weight: 50 kDa
Fluorescent image of MCF-7 cells stained with GATA3 antibody at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Flow cytometric analysis of MCF-7 cells using GATA3 antibody (green) and an isotype control of mouse IgG2b (blue); Ab was diluted at 1:25.

Documents

Further Information

Application Details :
Flow Cytometry: 1:25,Immunofluorescence: 1:25,Western blot: 1:1000
Application Note:
Titration of the GATA3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This GATA3 antibody was produced from a mouse immunized with a recombinant protein from human GATA3.
Limitation:
This GATA3 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P23771