Caveolin-1 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ4919
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4919-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 12C7
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Caveolin-1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 8
Immunofluorescent staining of FFPE human lung cancer tissue with Caveolin-1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 8
Immunofluorescent staining of FFPE human colorectal cancer tissue with Caveolin-1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
3 / 8
IHC staining of FFPE human melanoma with Caveolin-1antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 8
IHC staining of FFPE human intestinal cancer with Caveolin-1antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 8
IHC staining of FFPE human lung cancer with Caveolin-1antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 8
IHC staining of FFPE human intestinal cancer with Caveolin-1antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 8
IHC staining of frozen human placenta with Caveolin-1antibody.
8 / 8
Western blot testing of human 1) HeLa, 2) A549, 3) placenta, 4) U-87 MG, 5) PC-3 and 6) U-2 OS lysate with Calveolin-1 antibody. Predicted molecular weight ~21 kDa.
❮❯

Immunofluorescent staining of FFPE human lung cancer tissue with Caveolin-1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human colorectal cancer tissue with Caveolin-1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human melanoma with Caveolin-1antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human intestinal cancer with Caveolin-1antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with Caveolin-1antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human intestinal cancer with Caveolin-1antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of frozen human placenta with Caveolin-1antibody.
Western blot testing of human 1) HeLa, 2) A549, 3) placenta, 4) U-87 MG, 5) PC-3 and 6) U-2 OS lysate with Calveolin-1 antibody. Predicted molecular weight ~21 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence: 2-4ug/ml
Application Note:
Optimal dilution of the Caveolin-1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
CAV1( Caveolin-1) is a protein that in humans is encoded by the CAV1 gene. The CAV1 gene is mapped to 7q31.2. The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms(alpha and beta) are encoded by a single transcript from this gene.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids G4-I178 from the human protein were used as the immunogen for the Caveolin-1 antibody.
Limitation:
This Caveolin-1 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
Q03135