ATP5MC1/2/3 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ6294
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6294-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 12E9.
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the ATP5MC1/2/3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human HepG2 cells with ATP5MC1/2/3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATP5MC1/2/3 antibody.
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Flow cytometry testing of mouse HEPA1-6 cells with ATP5MC1/2/3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATP5MC1/2/3 antibody.
3 / 4
Flow cytometry testing of rat RH35 cells with ATP5MC1/2/3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATP5MC1/2/3 antibody.
4 / 4
Western blot testing of 1) human HL60, 2) monkey COS-7, 3) rat kidney, 4) rat heart and 5) mouse heart lysate with ATP5MC1/2/3 antibody. Predicted molecular weight: 10-14 kDa.
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Flow cytometry testing of human HepG2 cells with ATP5MC1/2/3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATP5MC1/2/3 antibody.
Flow cytometry testing of mouse HEPA1-6 cells with ATP5MC1/2/3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATP5MC1/2/3 antibody.
Flow cytometry testing of rat RH35 cells with ATP5MC1/2/3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATP5MC1/2/3 antibody.
Western blot testing of 1) human HL60, 2) monkey COS-7, 3) rat kidney, 4) rat heart and 5) mouse heart lysate with ATP5MC1/2/3 antibody. Predicted molecular weight: 10-14 kDa.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the ATP5MC1/2/3 antibody should be determined by the researcher.
Description:
The ATP5MC1 gene is one of three human paralogs that encode membrane subunit c of the mitochondrial ATP synthase. It is mapped to 17q21.32. This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene is one of three genes that encode subunit c of the proton channel. Each of the three genes have distinct mitochondrial import sequences but encode the identical mature protein. Alternatively spliced transcript variants encoding the same protein have been identified.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids D62-L113) was used as the immunogen for the ATP5MC1/2/3 antibody.
Limitation:
This ATP5MC1/2/3 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat, Monkey
Uniprot #:
P05496