Nucleolin Antibody

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NSJ Bioreagents
Product Code: NSJ-F40212
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F40212-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40212-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 130CT43.8.5
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the Nucleolin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of Nucleolin antibody and 293 lysate. Predicted molecular weight is 77 kDa, observed size is 100~110 kDa.
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Nucleolin antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle.
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Confocal immunofluorescent analysis of Nucleolin antibody with 293 cells followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used as a nuclear counterstain (blue).
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Western blot analysis of Nucleolin antibody and 293 lysate. Predicted molecular weight is 77 kDa, observed size is 100~110 kDa.
Nucleolin antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle.
Confocal immunofluorescent analysis of Nucleolin antibody with 293 cells followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used as a nuclear counterstain (blue).

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the Nucleolin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This Nucleolin antibody was produced from mice immunized with a protein from human NCL.
Limitation:
This Nucleolin antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P19338