SP1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F52486
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F52486-0.08ML0.08 ml£260.00
Quantity:
NSJ-F52486-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, k
Antibody Clonality: Monoclonal
Antibody Clone: 1326CT463.109.176
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the SP1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Western blot analysis of lysate from (1) HeLa and (2) Ramos cell line using SP1 antibody at 1:1000. Reported molecular weight: 81-95 kDa
2 / 3
Fluorescent image of HeLa cells stained with SP1 antibody at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
3 / 3
Flow cytometric analysis of HeLa cells using SP1 antibody (green) and an isotype control of mouse IgG1 (blue); Ab was diluted at 1:25 dilution.
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Western blot analysis of lysate from (1) HeLa and (2) Ramos cell line using SP1 antibody at 1:1000. Reported molecular weight: 81-95 kDa
Fluorescent image of HeLa cells stained with SP1 antibody at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Flow cytometric analysis of HeLa cells using SP1 antibody (green) and an isotype control of mouse IgG1 (blue); Ab was diluted at 1:25 dilution.

Documents

Further Information

Application Details :
Flow Cytometry: 1:25,Immunofluorescence: 1:25,Western blot: 1:1000
Application Note:
Titration of the SP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Specificity Protein 1/SP1 is a transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR- alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression. [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This SP1 antibody was produced from a mouse immunized with recombinant Specificity Protein 1.
Limitation:
This SP1 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P08047