PPAR alpha Antibody / PPARA

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NSJ Bioreagents
Product Code: NSJ-F54334
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54334-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54334-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 1331CT894.186.143
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the PPARA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
IHC testing of FFPE human skeletal muscle tissue with PPARA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 5
IHC testing of FFPE human kidney tissue with PPARA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 5
Immunofluorescent staining of fixed and permeabilized human HeLa cells with PPARA antibody (green) and anti-Actin (red).
4 / 5
Western blot testing of 1) human HeLa, 2) human Jurkat and 3) mouse NIH 3T3 cell lysate with PPARA antibody. Predicted molecular weight ~52 kDa.
5 / 5
Flow cytometry testing of fixed and permeabilized human HeLa cells with PPARA antibody; Blue=isotype control, Green= PPARA antibody.
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IHC testing of FFPE human skeletal muscle tissue with PPARA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human kidney tissue with PPARA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human HeLa cells with PPARA antibody (green) and anti-Actin (red).
Western blot testing of 1) human HeLa, 2) human Jurkat and 3) mouse NIH 3T3 cell lysate with PPARA antibody. Predicted molecular weight ~52 kDa.
Flow cytometry testing of fixed and permeabilized human HeLa cells with PPARA antibody; Blue=isotype control, Green= PPARA antibody.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells),Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the PPARA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl- 2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A recombinant human protein was used as the immunogen for the PPARA antibody.
Limitation:
This PPARA antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity
Species Reactivity :
Human, Mouse
Uniprot #:
Q07869