MMP14 Antibody

NSJ Bioreagents
Product Code: NSJ-F40218
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F40218-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40218-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgM
Antibody Clonality: Monoclonal
Antibody Clone: 133CT15.10.5.1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the MMP14 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of MMP14 antibody at 1:1000 dilution + HT-1080 lysate; Predicted band size : 66 kDa.
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Western blot testing of MMP14 antibody at 1:500 dilution + A2058 lysate; Predicted band size : 66 kDa.
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MMP14 antibody western blot analysis in A2058 lysate
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Confocal immunofluorescent analysis of MMP14 antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used as a nuclear counterstain (blue).

Western blot testing of MMP14 antibody at 1:1000 dilution + HT-1080 lysate; Predicted band size : 66 kDa.
Western blot testing of MMP14 antibody at 1:500 dilution + A2058 lysate; Predicted band size : 66 kDa.
MMP14 antibody western blot analysis in A2058 lysate
Confocal immunofluorescent analysis of MMP14 antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used as a nuclear counterstain (blue).

Further Information

Application Details :
Western blot: 1:100-1:1000,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the MMP14 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 470-499 from the human protein was used as the immunogen for this MMP14 antibody.
Limitation:
This MMP14 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat, Bovine, Pig, Rabbit
Uniprot #:
P50281